Combinational medicine
Background:
Nonsteroidal Anti-inflammatory Drugs (NSAIDs) such as Aspirin, have been introduced to the world for at least 2400 years. However, side effects such as stomach ulcers, stomach bleeding, and worsening asthma have limited the usage. Meanwhile, the root of Ophiopogon Japonicus (OPJ) has existed as one kind of Traditional Chinese Medicine (TCM) for even longer periods, while its anti-inflammatory effects have continuously been reported among people, but haven't been introduced and studied formally.
Purpose:
The study aimed to learn combined effects of anti-inflammatory constitutions of Ophiopogon Japonicus on lipopolysaccharide (LPS) induced RAW264.7 macrophage cells of mice.
Contents:
The main contents could be divided into tow parts:
Firstly, look for active anti-inflammatory constitutions of OPJ. The process was conduced as firstly extract Polysaccharide, Sapon, and other hydrophobic constitutions. After that, in vivo model were established by treating grouped mouse with stomach perfusion. Immune organism such as spleen was observed to see the inflammatory response.
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Based on the results that sapon extraction had better anti-inflammatory effect than glucose, we secondly designed to explore Ophiopogonin D (OP-D) and Ruscogenin (RUS) joint anti-inflammatory effects.​
To make result specifically, as well as considering the limit precision of extraction, we used in vitro cell model and commercial extractions. Cell viability were determined by MTT assay, and inflammatory states were noted by iNOs, to confirm the stability of the inflammatory model. Based on this, single and multiple drugs were added to the model, followed by a series of anti-inflammatory cytokines such as Interleukin 10 detection and analysis.
Conclusions:
The results showed that a single administration of OP-D, RUS,OJP 2,4,5 significantly decreased NO expression, inhibited the release of TNF-a and activated IL-10 release, showing a good dose-dependent(p<0.05). The combined effect of OP-D,RUS suggested that the two components could accelerate with each other. The cooperation of OJP-2 and OJP-4 could also be found, establishing on a good dose-dependent (p<0.5).